Proteomics and (bio-)pharmaceutical applications

The novel stationary phases and separation methods developed in the department are applied to successfully tackle the truly complex separation problems arising from proteomics and (bio-) pharmaceuticals research.

Novel performance limits have been established for the 1D-LC separation of complex peptide samples using monolithic column technology and coupled columns packed with core-shell particles. In addition, the separation of intact proteins, including protein isoforms arising from various amino-acid modifications, has been demonstrated employing a poly(styrene-co-divinylbenzene) monolithic capillary column in high-performance liquid chromatography coupled on-line to a time-of-flight mass spectrometer (TOF-MS).

Selected reading

J. De Vos, C. Stassen, A. Vaast, G. Desmet, S. Eeltink; High-resolution separations of tryptic digest mixtures using core-shell particulate columns operated at 1200 bar (J. Chromatogr. A, 2012, 1264, 57-62).

A. Vaast, J. De Vos, K. Broeckhoven, M. Verstraeten, S. Eeltink, G. Desmet; Maximizing the peak capacity using coupled columns packed with 2.6 µm core–shell particles operated at 1200 bar (J. Chromatogr. A 2012, 1256, 72-79).

S. Eeltink, B. Wouters, G. Desmet, M. Ursem, D. Blinco, G.D. Kemp, A. Treumann; High-resolution separations of protein isoforms with LC-TOF-MS using polymer monolithic capillary columns (J. Chromatogr. A, 2011, 32, 5504-5511).

Involved Staff: Gert, Bas, Ken, Jelle